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<!-- DFIN New ActiveDisclosure (SM) XBRL Linkbase Document - http://www.dfinsolutions.com/ -->
<!-- Creation Date :2023-04-27T12:49:06.9873+00:00 -->
<!-- Copyright (c) 2023 Donnelly Financial Solutions, Inc. All Rights Reserved. -->
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  <link:roleRef roleURI="http://ascendispharma.com/20230331/taxonomy/role/ConsolidatedCashFlowStatements" xlink:href="asnd-20230331.xsd#ConsolidatedCashFlowStatements" xlink:type="simple"/>
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